Dataset Search

This dataset was developed as part of an online survey study that explores student characteristics that may predict what one finds helpful in replies to requests for help posted to an online college course discussion forum. 223 college students enrolled in an introductory statistics course were surveyed on their sense of belonging to their course community, as well as how helpful they found 20 examples of replies to requests for help posted to a statistics course discussion forum.

A set of species entity mentions derived from an NERC dataset analyzing 900 synthetic biology articles published by the ACS. This data is associated with the Synthetic Biology Knowledge System repository (https://web.synbioks.org/). The data in this dataset are raw mentions from the NERC data.

Related to the raw entity mentions, this dataset represents the effects of the data cleaning process and collates all of the entity mentions which were too ambiguous to successfully link to the NCBI's taxonomy identifier system.

This dataset represents the results of manual cleaning and annotation of the entity mentions contained in the raw dataset (https://doi.org/10.13012/B2IDB-4950847_V1). Each mention has been consolidated and linked to an identifier for a matching concept from the NCBI's taxonomy database.

Compositional characterization of biomass is vital for the biofuel industry. Traditional wet chemistry-based methods for analyzing biomass composition are laborious, time-consuming, and require extensive use of chemical reagents as well as highly skilled personnel. In this study, near-infrared (NIR) spectroscopy was used to quickly assess the composition of above-ground vegetative biomass from 113 diverse, photoperiod-sensitive, biomass-type sorghum (Sorghum bicolor) accessions cultivated under field conditions in Central Illinois. Biomass samples were analyzed using NIR spectra collected in the spectral range of 867–2536 nm, with their chemical compositions determined following the National Renewable Energy Laboratory (NREL) protocol. Advanced spectral pre-treatment and band selection techniques were utilized to develop calibration models using partial least squares regression (PLSR). The models’ effectiveness was assessed through cross-validation and independent data tests. The predictions for moisture, ash, extractives, glucan, xylan, acid-soluble lignin (ASL), acid-insoluble lignin (AIL), and total lignin were accurate and reliable, demonstrating the capability of NIR spectroscopy to provide rapid and precise characterization of sorghum biomass. The results demonstrated that NIR spectroscopy is an efficient tool for rapidly characterizing sorghum biomass, making it a sustainable option for screening desirable feedstock for biofuel or bioproduct production.

This is a dataset from a choice experiment survey on family forest landowner preferences for managing invasive species.

Oilcane is an engineered sugarcane with the ability to hyper-accumulate vegetative lipids. It is processed to obtain juice and bagasse as a potential substrate for the production of biofuels and biochemicals. The juice comprises solid particles that are separated as waste mud before the fermentation of the juice. In this study, the oilcane waste mud (OWM) generated from 1000 liters of oilcane juice was quantified and evaluated as a potential resource for recovering biobased waxes. Hexane and ethyl acetate were evaluated as two different solvents for extracting waxes from OWM followed by its purification using acetone. The extracted biobased wax samples were characterized for their chemical and thermal profiles which were then compared with commercial natural waxes. Detailed mass balance shows that 53.6 ± 2.6 kg (dry basis) of solid OWM gets generated upon processing 1000 L (~1068 kg) of oilcane juice. Hexane and ethyl acetate led to a crude wax yield of 25.6 ± 0.2% and 16.6 ± 0.4% (wt/wt, dry basis) respectively from OWM at the end of 8 h. The relative purification of the wax samples was reported in the range of 58%–65% (wt/wt). The purified OWM wax has a melting point of 74.7°C. The waste mud was valorized as a source of biobased waxes with characteristic chemical and thermal profiles comparable to commercial natural waxes (carnauba and beeswax). Considering the decline in the supply of petroleum wax in the future coupled with the switch to “greener” alternative products by consumers, OWM could be a valuable source of natural wax in the industrial sector reducing the dependence on petroleum waxes. Eventually, recovering biobased wax as a co-product from OWM would bring in an additional stream of revenue leading to the development of a zero-waste biorefinery based on bioenergy crops.

Conjugate photoelectron energy spectra derived from coincident FUV and radio measurements. These are outputs of simulations from the semi-empirical SAMI2-PE (Varney et al. 2012) for the night of January 4, 2020.

*Updates for this V3: added a few more records and rearranged the sequence of the tables in order to support our new paper "Evaluation of Indirect and Direct Scoring Methods to Relate Biochemical Soil Quality Indicators to Ecosystem Services" accepted by the Soil Science Society of America Journal. We summarize peer reviewed literature reporting associations between for three soil quality indicators (SQIs) (β-glucosidase (BG), fluorescein diacetate (FDA) hydrolysis, and permanganate oxidizable carbon (POXC)) and crop yield and greenhouse gas emissions. Peer-reviewed articles published between January of 1990 and May 2018 were searched using the Thomas Reuters Web of Science database (Thomas Reuters, Philadelphia, Pennsylvania) and Google Scholar to identify studies reporting results for: “β-glucosidase”, “permanganate oxidizable carbon”, “active carbon”, “readily oxidizable carbon”, or “fluorescein diacetate hydrolysis”, together with one or more of the following: “crop yield”, “productivity”, “greenhouse gas’, “CO2”, “CH4”, or “N2O”. Meta-data for records include the following descriptor variables and covariates useful for scoring function development: 1) identifying factors for the study site (location, duration of the experiment), 2) soil textural class, pH, and SOC, 3) depth of soil sampling, 4) units used in published works (i.e.: equivalent mass, concentration), 5) SQI abundances and measured ecosystem functions, and 6) summary statistics for correlation between SQIs and functions (yield and greenhouse gas emissions). *Note: Blank values in tables are considered unreported data.

The complete data for the publication "RNA helicase MOV10 suppresses fear memory and dendritic arborization and regulates microtubule dynamics in hippocampal neurons," excluding sequencing data deposited in GEO, is provided here.

Plant bioengineering is a time-consuming and labor-intensive process with no guarantee of achieving desired traits. Here, we present a fast, automated, scalable, high-throughput pipeline for plant bioengineering (FAST-PB) in maize (Zea mays) and Nicotiana benthamiana. FAST-PB enables genome editing and product characterization by integrating automated biofoundry engineering of callus and protoplast cells with single-cell matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). We first demonstrated that FAST-PB could streamline Golden Gate cloning, with the capacity to construct 96 vectors in parallel. Using FAST-PB in protoplasts, we found that PEG2050 increased transfection efficiency by over 45%. For proof-of-concept, we established a reporter-gene-free method for CRISPR editing and phenotyping via mutation of high chlorophyll fluorescence 136. We show that diverse lipids were enhanced up to 6-fold using CRISPR activation of lipid controlling genes. In callus cells, an automated transformation platform was employed to regenerate plants with enhanced lipid traits through introducing multigene cassettes. Lastly, FAST-PB enabled high-throughput single-cell lipid profiling by integrating MALDI-MS with the biofoundry, protoplast, and callus cells, differentiating engineered and unengineered cells using single-cell lipidomics. These innovations massively increase the throughput of synthetic biology, genome editing, and metabolic engineering and change what is possible using single-cell metabolomics in plants.

Data associated with the manuscript "Do rusty crayfish invasions affect water clarity in north temperate lakes?" by Daniel K. Szydlowski, Melissa K. Daniels, and Eric R. lARSON

This dataset provides estimates of total Irrigation Water Use (IWU) by crop, county, water source, and year for the Continental United States. Total irrigation from Surface Water Withdrawals (SWW), total Groundwater Withdrawals (GWW), and nonrenewable Groundwater Depletion (GWD) is provided for 20 crops and crop groups from 2008 to 2020 at the county spatial resolution. In total, there are nearly 2.5 million data points in this dataset (3,142 counties; 13 years; 3 water sources; and 20 crops). This dataset supports the paper by Ruess et al (2024) "Total irrigation by crop in the Continental United States from 2008 to 2020", Scientific Data, doi: 10.1038/s41597-024-03244-w When using, please cite as: Ruess, P.J., Konar, M., Wanders, N., and Bierkens, M.F.P. (2024) Total irrigation by crop in the Continental United States from 2008 to 2020, Scientific Data, doi: 10.1038/s41597-024-03244-w

The nonconventional yeast Issatchenkia orientalis has emerged as a potential platform microorganism for production of organic acids due to its ability to grow robustly under highly acidic conditions. However, lack of efficient genetic tools remains a major bottleneck in metabolic engineering of this organism. Here we report that the autonomously replicating sequence (ARS) from Saccharomyces cerevisiae (ScARS) was functional for plasmid replication in I. orientalis, and the resulting episomal plasmid enabled efficient genome editing by the CRISPR/Cas9 system. The optimized CRISPR/Cas9-based system employed a fusion RPR1′-tRNA promoter for single guide RNA (sgRNA) expression and could attain greater than 97% gene disruption efficiency for various gene targets. Additionally, we demonstrated multiplexed gene deletion with disruption efficiencies of 90% and 47% for double gene and triple gene knockouts, respectively. This genome editing tool can be used for rapid strain development and metabolic engineering of this organism for production of biofuels and chemicals.

School buses transport 20 million students annually and are currently undergoing electrification in the US. With Vehicle-to-Building (V2B) technology, electric school buses (ESBs) can supply energy to school buildings during power outages, ensuring continued operation and safety. This study proposes assessing the resilience of secondary schools during outages by leveraging ESB fleets as backup power across various US climate regions. The findings indicate that the current fleet of ESBs in representative cities across different climate regions in the US is insufficient to meet the power demands of an entire school or even its HVAC system. However, we estimated the number of ESBs required to support the school's power needs, and we showed that the use of V2B technology significantly reduces carbon emissions compared to backup diesel generators. While adjusting HVAC setpoints and installing solar panels have limited impacts on enhancing school resilience, gathering students in classrooms during outages significantly improved resilience in our case study in Houston, Texas. Given the ongoing electrification of school buses, it is essential for schools to complement ESBs with stationary batteries and other backup power sources, such as solar and/or diesel generators, to effectively address prolonged outages. Determining the deployment of direct current fast and Level 2 chargers can reduce infrastructure costs while maintaining the resilience benefits of ESBs. This dataset includes the simulation process and results of this study.

12CO and 13CO emission maps of the 30 Doradus molecular cloud in the Large Magellanic Cloud, obtained with the Atacama Large Millimeter/submillimeter Array (ALMA) during Cycle 7. See the associated article in the Astrophysical Journal, and README file, for details. Please cite the article if you use these data.

Dataset for "Spatial drivers of wetland bird occupancy within an urbanized matrix in the Upper Midwestern United States" manuscript contains occupancy data for ten wetland bird species used in single-species occupancy models at four spatial scales and four wetland habitat types. Data were collected from 2017-2019 in NE Illinois and NW Indiana. Dataset includes wetland bird occupancy data, habitat parameter values for each survey location, and R code used to run analyses.

Rhodotorula toruloides is a non-model, oleaginous yeast uniquely suited to produce acetyl-CoA-derived chemicals. However, the lack of well-characterized genomic integration sites has impeded the metabolic engineering of this organism. Here we report a set of computationally predicted and experimentally validated chromosomal integration sites in R. toruloides. We first implemented an in silico platform by integrating essential gene information and transcriptomic data to identify candidate sites that meet stringent criteria. We then conducted a full experimental characterization of these sites, assessing integration efficiency, gene expression levels, impact on cell growth, and long-term expression stability. Among the identified sites, 12 exhibited integration efficiencies of 50% or higher, making them sufficient for most metabolic engineering applications. Using selected high-efficiency sites, we achieved simultaneous double and triple integrations and efficiently integrated long functional pathways (up to 14.7 kb). Additionally, we developed a new inducible marker recycling system that allows multiple rounds of integration at our characterized sites. We validated this system by performing five sequential rounds of GFP integration and three sequential rounds of MaFAR integration for fatty alcohol production, demonstrating, for the first time, precise gene copy number tuning in R. toruloides. These characterized integration sites should significantly advance metabolic engineering efforts and future genetic tool development in R. toruloides.

Supplemental Forest Data for Chapter 6: Climate Change Impacts on Ecosystems in "An Assessment of the Impacts of Climate Change in Illinois"

This dataset was created based on the publicly available microdata from PNS-2019, a national health survey conducted by the Instituto Brasileiro de Geografia e Estatistica (IBGE, Brazilian Institute of Geography and Statistics). IBGE is a federal agency responsible for the official collection of statistical information in Brazil – essentially, the Brazilian census bureau. Data on selected variables focusing on biopsychosocial domains related to pain prevalence, limitations and treatment are available. The Fundação Instituto Oswaldo Cruz has detailed information about the PNS, including questionnaires, survey design, and datasets (www.pns.fiocruz.br). The microdata can be found on the IBGE website (https://www.ibge.gov.br/estatisticas/downloads-estatisticas.html?caminho=PNS/2019/Microdados/Dados).

- The aim of this research was to evaluate the novel dietary fiber source, miscanthus grass, in comparison to traditional fiber sources, and their effects on the microbiota of healthy adult cats. Four dietary treatments, cellulose (CO), miscanthus grass fiber (MF), a blend of miscanthus fiber and tomato pomace (MF+TP), or beet pulp (BP) were evaluated.<br /><br />- The study was conducted using a completely randomized design with twenty-eight neutered adult, domesticated shorthair cats (19 females and 9 males, mean age 2.2 ± 0.03 yr; mean body weight 4.6 ± 0.7 kg, mean body condition score 5.6 ± 0.6). Total DNA from fresh fecal samples was extracted using Mo-Bio PowerSoil kits (MO BIO Laboratories, Inc., Carlsbad, CA). Amplification of the 292 bp-fragment of V4 region from the 16S rRNA gene was completed using a Fluidigm Access Array (Fluidigm Corporation, South San Francisco, CA). Paired-end Illumina sequencing was performed on a MiSeq using v3 reagents (Illumina Inc., San Diego, CA) at the Roy J. Carver Biotechnology Center at the University of Illinois. <br />- Filenames are composed of animal name identifier, diet (BP= beet pulp; CO= cellulose; MF= miscanthus grass fiber; TP= blend of miscanthus fiber and tomato pomace).

An Atlas.ti dataset and accompanying documentation of a thematic analysis of problems and opportunities associated with retracted research and its continued citation.

This dataset includes mass spectrometry, library screening, and gas chromatography data used for creating a high-throughput screening in metabolic engineering.

The materials used to provide Continuing Medical Education on ticks and tick-borne diseases in Illinois on February 1, 2023 at Carle Hospital, along with the pre- and post-quiz and deidentified data of the quiz takers. Files: "Ticks and Tick-borne Diseases of Illinois_Final_w_speaker_notes.pptx": Presentation slides used for CME course, with notes to indicate verbal commentary "CME assessment_final.docx": Pre- and post-CME quiz questions and answers, annotated to indicate correct answers and reasoning for incorrect answers "CME_prequiz_data_for_sharing.csv": De-identified data from pre-CME quiz "CME_postquiz_data_for_sharing.csv": De-identified data from post-CME quiz, including demographics "DataCleaning_forSharing.R": R file used to clean the raw data and calculate the scores "ReadMe.txt":

Various works have quantitatively characterized the effects of environmental and management factors on Miscanthus x giganteus Greef et Deu (mxg) yield and, therefore, anticipated land requirement per unit production. However, little work has addressed the effects of cutting height, which may significantly contribute to the difference between the standing aboveground biomass at harvest (i.e., biological yield) and harvested yield. This study quantitatively characterized the effect of cutting height using a replicated nitrogen trial of a 5-year-old mxg stand in southeast Iowa and related this information to observations of cutting height in nearby commercial fields. Nitrogen fertilizer did not significantly change the relationship of the stem segment mass to length, and overall, a 1-cm stem segment contributes 0.5% of the total stem biomass within the bottom 44 cm of the stem. This results in an average harvest loss of 15% of the aboveground standing biomass when cutting at 30 cm, typically seen in commercial mxg fields in eastern Iowa. Cutting height should be considered when accurately predicting commercial mxg harvest yields and changes in soil organic carbon in a commercial mxg agroecosystem.